Research Articles
Biofluorescence vs Bioluminescence: Mechanisms, Applications, and Strategic Implementation in Biomedical Research
This article provides a comprehensive analysis of the fundamental mechanisms distinguishing biofluorescence and bioluminescence, two pivotal photonic phenomena in life sciences.
Indocyanine Green Fluorescence-Guided Surgery: From Fundamental Principles to Clinical Applications and Future Directions
This comprehensive review explores the rapidly evolving field of fluorescence-guided surgery (FGS) using indocyanine green (ICG), a near-infrared fluorophore.
NIR-II Fluorescence Imaging: Revolutionizing Deep-Tissue Visualization for Biomedical Research and Clinical Translation
This article comprehensively explores second near-infrared (NIR-II, 1000–1700 nm) fluorescence imaging, an advanced optical modality offering superior tissue penetration and high-fidelity visualization for preclinical research and emerging clinical applications.
Beyond Jellyfish: The Evolutionary Diversity and Biomedical Applications of GFP Analogs
This article provides a comprehensive exploration of the Green Fluorescent Protein (GFP) family, tracing its phylogenetic journey from its discovery in Aequorea victoria to the vast diversity of analogs found...
Correcting Wavelength-Dependent Light Attenuation in Tissue: Techniques, Applications, and Quantitative Validation for Biomedical Research
Accurate interpretation of optical signals in biological tissues is fundamentally challenged by wavelength-dependent attenuation from absorption and scattering.
Overcoming Penetration Depth Limits in Fluorescence Imaging: From NIR Probes to Clinical Solutions
This article provides a comprehensive analysis of the penetration depth limitations inherent to fluorescence imaging, a critical challenge for researchers and drug development professionals.
Strategies to Reduce Tissue Autofluorescence in NIR Imaging: A Guide for Enhanced Biomedical Research
Near-infrared (NIR) fluorescence imaging, particularly in the NIR-II window (1000–1700 nm), is revolutionizing biomedical research and drug development by enabling deep-tissue visualization with high resolution.
Targeted Nanoparticle Probes for Diagnostic Fluorescence Imaging: Advances, Applications, and Clinical Translation
This article provides a comprehensive overview of the latest advancements in targeted nanoparticle probes for diagnostic fluorescence imaging, tailored for researchers, scientists, and drug development professionals.
A Scientific Guide to Filter Selection for Underwater Biofluorescence Imaging in Biomedical Research
This article provides a comprehensive framework for researchers, scientists, and drug development professionals to select and implement optical filters for underwater biofluorescence photography.
Multiplexed Fluorescence Imaging: A Guide to High-Plex Biomarker Analysis for Drug Development and Clinical Research
This article provides a comprehensive overview of modern multiplexed fluorescence imaging, a transformative technology enabling the simultaneous visualization of numerous biomarkers within their native tissue context.