Research Articles
The Evolution of Anthozoan GFP Chromoproteins: From Coral Reefs to Biomedical Innovation
This article provides a comprehensive analysis of GFP (Green Fluorescent Protein) chromoprotein evolution within Anthozoa (corals and anemones), exploring its foundational biology, methodological applications in research, troubleshooting for experimental use,...
Illuminating Membrane Proteins: The Complete Guide to Using GFP as a Reporter for Expression Analysis
This comprehensive article provides a detailed roadmap for researchers and drug development professionals utilizing Green Fluorescent Protein (GFP) as a reporter for membrane protein expression.
FluorIS In Situ Imaging: Revolutionizing Coral Fluorescence Analysis for Biomedical Research
This article provides a comprehensive guide to the FluorIS system for in situ coral fluorescence imaging, a transformative technology with significant implications for biomedical and drug discovery research.
Firefly vs. NanoLuc Luciferase: A 2024 Guide to Brightness, Size, and Application in Biomedical Research
This comprehensive guide provides researchers, scientists, and drug development professionals with a detailed, up-to-date comparison of Firefly (Fluc) and NanoLuc (Nluc) luciferase reporter systems.
Unlocking Protein Stability: A Comprehensive Guide to FSEC-TS Thermostability Assays with GFP Fusion Proteins
This article provides a complete guide to the Fluorescence Size Exclusion Chromatography-Thermal Shift (FSEC-TS) assay for drug discovery and protein engineering researchers.
Mastering FRET Imaging with CFP-YFP Pairs: A Comprehensive Guide for Biomedical Research and Drug Discovery
This definitive guide provides biomedical researchers and drug development professionals with a comprehensive framework for implementing and optimizing FRET (Förster Resonance Energy Transfer) imaging using CFP (Cyan Fluorescent Protein) and...
FRET Efficiency Showdown: A Comparative Guide to FLIM Calculation Methods for Precision in Biomedical Research
This comprehensive guide provides biomedical researchers and drug development professionals with a detailed comparison of methods for calculating Förster Resonance Energy Transfer (FRET) efficiency using Fluorescence Lifetime Imaging Microscopy (FLIM).
Navigating the Oxidative Challenge: Optimizing Fluorescent Protein Performance in the Secretory Pathway for Advanced Cell Biology and Drug Discovery
This article provides a comprehensive guide for researchers on the critical factors influencing fluorescent protein (FP) stability and function within the oxidizing environment of the secretory pathway.
Monomeric FP Revolution: Overcoming Dimerization Artifacts in Live-Cell Imaging and Drug Discovery
Fluorescent protein (FP) dimerization has long been a major source of experimental artifacts in live-cell imaging, protein interaction studies, and biosensor design, leading to false positives and misleading biological interpretations.
FLISimBA: A Comprehensive Guide to Simulation-Based FLIM Experimental Design for Drug Discovery
This article provides a complete framework for leveraging the FLISimBA simulation tool to design robust Fluorescence Lifetime Imaging (FLIM) experiments, particularly for drug development research.